Widziałeś ten? Od http://www.aspspider.com/resources/Resource510.aspx :

public DataTable Import(String path)
{
    Microsoft.Office.Interop.Excel.Application app = new Microsoft.Office.Interop.Excel.Application();
    Microsoft.Office.Interop.Excel.Workbook workBook = app.Workbooks.Open(path, 0, true, 5, "", "", true, Microsoft.Office.Interop.Excel.XlPlatform.xlWindows, "\t", false, false, 0, true, 1, 0);

    Microsoft.Office.Interop.Excel.Worksheet workSheet = (Microsoft.Office.Interop.Excel.Worksheet)workBook.ActiveSheet;

    int index = 0;
    object rowIndex = 2;

    DataTable dt = new DataTable();
    dt.Columns.Add("FirstName");
    dt.Columns.Add("LastName");
    dt.Columns.Add("Mobile");
    dt.Columns.Add("Landline");
    dt.Columns.Add("Email");
    dt.Columns.Add("ID");

    DataRow row;

    while (((Microsoft.Office.Interop.Excel.Range)workSheet.Cells[rowIndex, 1]).Value2 != null)
    {

        row = dt.NewRow();
        row[0] = Convert.ToString(((Microsoft.Office.Interop.Excel.Range)workSheet.Cells[rowIndex, 1]).Value2);
        row[1] = Convert.ToString(((Microsoft.Office.Interop.Excel.Range)workSheet.Cells[rowIndex, 2]).Value2);
        row[2] = Convert.ToString(((Microsoft.Office.Interop.Excel.Range)workSheet.Cells[rowIndex, 3]).Value2);
        row[3] = Convert.ToString(((Microsoft.Office.Interop.Excel.Range)workSheet.Cells[rowIndex, 4]).Value2);
        row[4] = Convert.ToString(((Microsoft.Office.Interop.Excel.Range)workSheet.Cells[rowIndex, 5]).Value2);
        index++;

        rowIndex = 2 + index;
        dt.Rows.Add(row);
    }
    app.Workbooks.Close();
    return dt;
}

object[,] valueArray = (object[,])excelRange.get_Value(XlRangeValueDataType.xlRangeValueDefault);

//Get the column names
for (int k = 0; k < valueArray.GetLength(1); )
{
    //add columns to the data table.
    dt.Columns.Add((string)valueArray[1,++k]);
}

//Load data into data table
object[] singleDValue = new object[valueArray.GetLength(1)];
//value array first row contains column names. so loop starts from 1 instead of 0
for (int i = 1; i < valueArray.GetLength(0); i++)
{
    Console.WriteLine(valueArray.GetLength(0) + ":" + valueArray.GetLength(1));
    for (int k = 0; k < valueArray.GetLength(1); )
    {
        singleDValue[k] = valueArray[i+1, ++k];
    }
    dt.LoadDataRow(singleDValue, System.Data.LoadOption.PreserveChanges);
}

using System;
using System.Collections.Generic;
using System.Linq;
using System.Text;
using System.Data;
using System.Reflection;
using Microsoft.Office.Interop.Excel;

namespace trg.satmap.portal.ParseAgentSkillMapping
{
    class ConvertXLStoDT
    {
        private StringBuilder errorMessages;

        public StringBuilder ErrorMessages
        {
            get { return errorMessages; }
            set { errorMessages = value; }
        }

        public ConvertXLStoDT()
        {
            ErrorMessages = new StringBuilder();
        }

        public System.Data.DataTable XLStoDTusingInterOp(string FilePath)
        {
            #region Excel important Note.
            /*
             * Excel creates XLS and XLSX files. These files are hard to read in C# programs. 
             * They are handled with the Microsoft.Office.Interop.Excel assembly. 
             * This assembly sometimes creates performance issues. Step-by-step instructions are helpful.
             * 
             * Add the Microsoft.Office.Interop.Excel assembly by going to Project -> Add Reference.
             */
            #endregion

            Microsoft.Office.Interop.Excel.Application excelApp = null;
            Microsoft.Office.Interop.Excel.Workbook workbook = null;


            System.Data.DataTable dt = new System.Data.DataTable(); //Creating datatable to read the content of the Sheet in File.

            try
            {

                excelApp = new Microsoft.Office.Interop.Excel.Application(); // Initialize a new Excel reader. Must be integrated with an Excel interface object.

                //Opening Excel file(myData.xlsx)
                workbook = excelApp.Workbooks.Open(FilePath, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value, Missing.Value);

                Microsoft.Office.Interop.Excel.Worksheet ws = (Microsoft.Office.Interop.Excel.Worksheet)workbook.Sheets.get_Item(1);

                Microsoft.Office.Interop.Excel.Range excelRange = ws.UsedRange; //gives the used cells in sheet

                ws = null; // now No need of this so should expire.

                //Reading Excel file.               
                object[,] valueArray = (object[,])excelRange.get_Value(Microsoft.Office.Interop.Excel.XlRangeValueDataType.xlRangeValueDefault);

                excelRange = null; // you don't need to do any more Interop. Now No need of this so should expire.

                dt = ProcessObjects(valueArray);                

            }
            catch (Exception ex)
            {
                ErrorMessages.Append(ex.Message);
            }
            finally
            {
                #region Clean Up                
                if (workbook != null)
                {
                    #region Clean Up Close the workbook and release all the memory.
                    workbook.Close(false, FilePath, Missing.Value);                    
                    System.Runtime.InteropServices.Marshal.ReleaseComObject(workbook);
                    #endregion
                }
                workbook = null;

                if (excelApp != null)
                {
                    excelApp.Quit();
                }
                excelApp = null;                

                #endregion
            }
            return (dt);
        }

        /// <summary>
        /// Scan the selected Excel workbook and store the information in the cells
        /// for this workbook in an object[,] array. Then, call another method
        /// to process the data.
        /// </summary>
        private void ExcelScanIntenal(Microsoft.Office.Interop.Excel.Workbook workBookIn)
        {
            //
            // Get sheet Count and store the number of sheets.
            //
            int numSheets = workBookIn.Sheets.Count;

            //
            // Iterate through the sheets. They are indexed starting at 1.
            //
            for (int sheetNum = 1; sheetNum < numSheets + 1; sheetNum++)
            {
                Worksheet sheet = (Worksheet)workBookIn.Sheets[sheetNum];

                //
                // Take the used range of the sheet. Finally, get an object array of all
                // of the cells in the sheet (their values). You can do things with those
                // values. See notes about compatibility.
                //
                Range excelRange = sheet.UsedRange;
                object[,] valueArray = (object[,])excelRange.get_Value(XlRangeValueDataType.xlRangeValueDefault);

                //
                // Do something with the data in the array with a custom method.
                //
                ProcessObjects(valueArray);
            }
        }
        private System.Data.DataTable ProcessObjects(object[,] valueArray)
        {
            System.Data.DataTable dt = new System.Data.DataTable();

            #region Get the COLUMN names

            for (int k = 1; k <= valueArray.GetLength(1); k++)
            {
                dt.Columns.Add((string)valueArray[1, k]);  //add columns to the data table.
            }
            #endregion

            #region Load Excel SHEET DATA into data table

            object[] singleDValue = new object[valueArray.GetLength(1)];
            //value array first row contains column names. so loop starts from 2 instead of 1
            for (int i = 2; i <= valueArray.GetLength(0); i++)
            {
                for (int j = 0; j < valueArray.GetLength(1); j++)
                {
                    if (valueArray[i, j + 1] != null)
                    {
                        singleDValue[j] = valueArray[i, j + 1].ToString();
                    }
                    else
                    {
                        singleDValue[j] = valueArray[i, j + 1];
                    }
                }
                dt.LoadDataRow(singleDValue, System.Data.LoadOption.PreserveChanges);
            }
            #endregion


            return (dt);
        }
    }
}

Cicho późno!.

Ta metoda jest poprawnie przetestowana i konwertuje Excela do DataSet.

public DataSet Dtl()
        {
            //Instance reference for Excel Application
            Microsoft.Office.Interop.Excel.Application objXL = null;        
            //Workbook refrence
            Microsoft.Office.Interop.Excel.Workbook objWB = null;
            DataSet ds = new DataSet();
            try
            {
                objXL = new Microsoft.Office.Interop.Excel.Application();
                objWB = objXL.Workbooks.Open(@"Book1.xlsx");//Your path to excel file.
                foreach (Microsoft.Office.Interop.Excel.Worksheet objSHT in objWB.Worksheets)
                {
                    int rows = objSHT.UsedRange.Rows.Count;
                    int cols = objSHT.UsedRange.Columns.Count;
                    DataTable dt = new DataTable();
                    int noofrow = 1;
                    //If 1st Row Contains unique Headers for datatable include this part else remove it
                    //Start
                    for (int c = 1; c <= cols; c++)
                    {
                        string colname = objSHT.Cells[1, c].Text;
                        dt.Columns.Add(colname);
                        noofrow = 2;
                    }
                    //END
                    for (int r = noofrow; r <= rows; r++)
                    {
                        DataRow dr = dt.NewRow();
                        for (int c = 1; c <= cols; c++)
                        {
                            dr[c - 1] = objSHT.Cells[r, c].Text;
                        }
                        dt.Rows.Add(dr);
                    }
                   ds.Tables.Add(dt);
                }
                //Closing workbook
                objWB.Close();
                //Closing excel application
                objXL.Quit();
                return ds;
            }

            catch (Exception ex)
            {
               objWB.Saved = true;
                //Closing work book
                objWB.Close();
                //Closing excel application
                objXL.Quit();
                //Response.Write("Illegal permission");
                return ds;
            }
        }

Lata po odpowiedzi wszystkich, ja również chcę przedstawić, jak zrobiłem to dla mojego projektu

    /// <summary>
    /// /Reads an excel file and converts it into dataset with each sheet as each table of the dataset
    /// </summary>
    /// <param name="filename"></param>
    /// <param name="headers">If set to true the first row will be considered as headers</param>
    /// <returns></returns>
    public DataSet Import(string filename, bool headers = true)
    {
        var _xl = new Excel.Application();
        var wb = _xl.Workbooks.Open(filename);
        var sheets = wb.Sheets;
        DataSet dataSet = null;
        if (sheets != null && sheets.Count != 0)
        {
            dataSet = new DataSet();
            foreach (var item in sheets)
            {
                var sheet = (Excel.Worksheet)item;
                DataTable dt = null;
                if (sheet != null)
                {
                    dt = new DataTable();
                    var ColumnCount = ((Excel.Range)sheet.UsedRange.Rows[1, Type.Missing]).Columns.Count;
                    var rowCount = ((Excel.Range)sheet.UsedRange.Columns[1, Type.Missing]).Rows.Count;

                    for (int j = 0; j < ColumnCount; j++)
                    {
                        var cell = (Excel.Range)sheet.Cells[1, j + 1];
                        var column = new DataColumn(headers ? cell.Value : string.Empty);
                        dt.Columns.Add(column);
                    }

                    for (int i = 0; i < rowCount; i++)
                    {
                        var r = dt.NewRow();
                        for (int j = 0; j < ColumnCount; j++)
                        {
                            var cell = (Excel.Range)sheet.Cells[i + 1 + (headers ? 1 : 0), j + 1];
                            r[j] = cell.Value;
                        }
                        dt.Rows.Add(r);
                    }

                }
                dataSet.Tables.Add(dt);
            }
        }
        _xl.Quit();
        return dataSet;
    }